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Table 2 Sequences of peptides used for the ELISA experiments and for the bifunctional nanoconstructs

From: In vitro eradication of citrullinated protein specific B-lymphocytes of rheumatoid arthritis patients by targeted bifunctional nanoparticles

Peptides

Code

Amino acid sequencea

Fibrinβ60–74

β60-74Arg

H-60RPAPPPISGGGYRAR74-NH2

Fibrinβ60–74Cit60,72,74

β60-74Cit

H-60 XPAPPPISGGGYXAX 74-NH2

Biot-fibrinβ60–74

Biot-β60-74Arg

Biot-60RPAPPPISGGGYRAR74−NH2

Biot-fibrinβ60–74Cit60,72,74

Biot-β60-74Cit

Biot-60 XPAPPPISGGGYXAX 74-NH2

HIV-1 gp120233–247 Complement activating peptide

CNNK

Ac-233C(Acm)NNKTFNGTGPC(Acm)TNV247-NH2

HIV-1 gp120233–247 Q-substituted complement activating peptide

CNNQ

Ac-233C(Acm)NNQTFNGTGPC(Acm)TNV247-NH2

HIV-1 gp120233–247 Q-substituted complement activating peptide with C-terminal K

CNNQK

Ac-233C(Acm)NNQTFNGTGPC(Acm)TNV247-K-NH2

  1. aStandard one-letter code for amino acid residues: X citrulline, Ac– acetyl group, –NH 2 amid group, Acm acetamidomethyl group, Biot biotinyl-aminohexanoyl group
  2. ELISA enzyme-linked immunosorbent assay, HIV-1 human immunodeficiency virus type 1
  3. Bold letters stand for modified amino acids that are not present in the natural sequences
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Arthritis Research & Therapy

ISSN: 1478-6362

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