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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Effect of M3 muscarinic acetylcholine receptor deficiency on collagen antibody-induced arthritis

Fig. 5

Effect of M3R-deficiency on pro-inflammatory cytokine IL-6 in mice with CAIA. a Enzyme-linked immunosorbent assay (ELISA) measurement of IL-6 concentration in plasma of male arthritic WT and M3R−/− mice and respective LPS-treated control mice. b Correlation of circulating IL-6 abundance with the respective cumulative arthritis score (CAS) of arthritic WT and M3R−/− mice. Dotted lines indicate the mean IL-6 concentration of the respective LPS-treated control mice. c Ratio of plasma IL-6 level and CAS in arthritic male WT and M3R−/− mice given as mean ± SEM. # P < 0.05: M3R−/−-CAIA vs. WT-CAIA. d Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis of Il6 mRNA expression in paws of male WT and M3R−/− mice with CAIA and respective LPS-treated control mice, normalized to β-actin mRNA. **P < 0.01 CAIA vs. respective LPS control. e ELISA measurement of IL-6 concentration in paw homogenates of male LPS-treated and arthritic WT and M3R−/− mice. Data were normalized to total protein concentration. *P < 0.05 and **P < 0.01 CAIA vs. respective LPS control. CAIA collagen antibody-induced arthritis, CAS cumulative arthritis score, IL-6 interleukin-6, LPS lipopolysaccharide, M3R M3 muscarinic acetylcholine receptor, WT wild-type

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