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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Anti-TNF certolizumab pegol induces antioxidant response in human monocytes via reverse signaling

Fig. 1

Certolizumab pegol (CZP) induces rapid production of reactive oxygen species (ROS) and nuclear translocation of nuclear factor (erythroid-derived 2)-like (Nrf2). Monocytes were incubated, or not, with CZP (5 μg/ml) and ROS production was assessed by H2DCFCA using flow cytometry. The time course of rapid ROS production over 30 minutes is shown in monocytes from four different healthy donors (a). Monocytes were incubated, or not, with CZP (5 μg/ml) for 90 minutes, and cellular localization of Nrf2 was analyzed by wide-field microscopy using an anti-Nrf2 antibody. 4',6-diamidino-2-phenylindole (DAPI) was used as control for nucleus staining (b). Cytoplasmic and nuclear extracts were prepared after 90 minutes of cell culture and analyzed for Nrf2 detection (c). Time course analysis of Nrf2 translocation in nuclear fraction-enriched cell extracts of monocytes incubated, or not, with CZP (5 µg/ml) (d). TATA box binding protein (TBP) was used as control (d). Data are representative of four experiments (b, c, d). MFI geometric mean of fluorescence intensity

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