Fig. 4
From: Anti-TNF certolizumab pegol induces antioxidant response in human monocytes via reverse signaling
Heme oxygenase 1 (HO-1) induction by anti-TNF certolizumab pegol (CZP) is due to reverse signaling. Monocytes were incubated, or not, with CZP (5 μg/ml) for 16 h in the presence of TNF receptor 1 (TNFR1) and TNFR2 (5 μg/ml) blocking antibody. HO-1 protein expression was assessed by western blot (a). Quantification of four western blot experiments was performed. Paired t test p = 0.05 (b). GAPDH glyceraldehyde 3-phosphate dehydrogenase, Abs antibodies