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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Anti-TNF certolizumab pegol induces antioxidant response in human monocytes via reverse signaling

Fig. 5

Certolizumab pegol (CZP) directly induces inhibition of the toll-like receptor 4 pathway. Expression of IL1β: monocytes were incubated, or not, with CZP (5 μg/ml) for 1 h, washed, then stimulated with lipopolysaccharide (LPS) (10 ng/ml) for 16 h. IL1β mRNA was assessed by qRT-PCR. Results are expressed as relative level of IL1β mRNA mean ± SD in three experiments (a). IL1β protein was assessed by western blot in monocytes stimulated as in (a) for 16 h (b). Data shown are representative of three experiments. Monocytes were incubated, or not, with CZP (5 μg/ml) for 1 h, washed and stimulated with LPS (10 ng/ml) for 16 h. The level of ROS production was expressed as the percentage of variation of the geometric mean fluorescence intensity (MFI) of H2DFCDA as analyzed by flow cytometry (c) in five experiments. *Paired t test <0.05 for comparison of LPS and LPS + CZP. A representative cytometry experiment in shown (d). GAPDH glyceraldehyde 3-phosphate dehydrogenase

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