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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Generation mechanism of RANKL+ effector memory B cells: relevance to the pathogenesis of rheumatoid arthritis

Fig. 3

CXCR3+RANKL+ B cells accumulate in synovial fluid (SF) of patients with rheumatoid arthritis (SFRA). a CD80 and CD86 expression was analyzed in SF B cells from RA patients (n = 8). Receptor activator of nuclear factor kappa-B ligand (RANKL) expression in CD80–CD86– and CD80+CD86+ SF B cells was analyzed by flow cytometry. b The proportion of naïve (IgD+CD27–), IgD+-memory (IgD + -m, IgD+CD27+), double-negative (DN) and switched-memory (Sw-m, IgD–CD27+) B cells in SF, and RANKL expression of each subset was analyzed (n = 8). c The proportion of CXCR3+RANKL+ B cells was analyzed in peripheral blood of healthy controls (PBHC, n = 13), peripheral blood of patients with RA (PBRA, n = 18) and SFRA (n = 8). CXCR3+ and CXCR3– SF B cells from patients with RA were analyzed for mean fluorescence intensity (MFI) of RANKL expression by flow cytometry (n = 8, *P < 0.05). a–c Box plots represent the median values, quartiles and extremes (*P < 0.05, **P < 0.005). Representative data are shown. NS not significant

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