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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Increased caveolin-1 in intervertebral disc degeneration facilitates repair

Fig. 6

The effect of in vitro caveolin (Cav)-1 silencing on chondrocyte-like cells (CLCs) from chondrodystrophic dogs. In vitro Cav-1 silencing decreased transforming growth factor-β/Smad2/3 signaling and canine CLC extracellular matrix deposition, while 10 μM Cav-1 scaffolding domain peptide (CSD) supplementation rescued this effect. a Cav-1 messenger RNA (n-fold change ± SD) and protein knockdown by microaggregates silenced for Cav-1 (siRNA). The Cav-1 protein level in the mock-treated microaggregates at day 7 was set at 100 %. b Real-time quantitative polymerase chain reaction results (n-fold change ± SD). Gene expression in the nontreated CLC microaggregates was set at 1. c DNA and glycosaminoglycan (GAG) content (corrected for DNA content) of the canine CLC microaggregates with corresponding Safranin O/Fast Green, collagen type I, alpha 1 (Col I), and collagen type II, alpha 1 (Col II) immunohistochemically stained sections. Bars indicate 100 μm. n = 6. ACAN aggrecan, BCL2 B-cell CLL/lymphoma 2, ALK5 activin receptor-like kinase 5, PAI1 plasminogen activator inhibitor 1, ADAMTS5 a disintegrin and metalloproteinase with thrombospondin motifs 5, MMP13 matrix metalloproteinase 13. *p < 0.05, **p < 0.01

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