Fig. 7
From: Increased caveolin-1 in intervertebral disc degeneration facilitates repair
The dose–response effect of caveolin-1 on canine and human chondrocyte-like cells (CLCs) in vitro. DNA content, glycosaminoglycan (GAG) content (corrected for DNA content), and GAG release into the culture medium of human and chondrodystrophic (CD) and nonchondrodystrophic (NCD) canine CLC microaggregates with corresponding Safranin O (Saf O)/Fast Green collagen types I (Col I) and II (Col II) immunohistochemically stained sections. Generally, the GAG content was increased in the microaggregates treated with 25 μM caveolin-1 scaffolding domain (CSD), whereas the GAG release in the culture medium was decreased in these 25 μM CSD-treated microaggregates. T 2 2 ng/ml transforming growth factor (TGF)-β1, T 10 10 ng/ml TGF-β1, C 10 10 μM CSD, C 25 25 μM CSD. Bars indicate 100 μm. n = 5 (CD and NCD) and n = 3 (human). Two microaggregates were analyzed per donor and condition. *p < 0.05