Fig. 1
From: Nicotinic acetylcholine receptors modulate osteoclastogenesis
Osteoclast formation is inhibited by nAChR ligands in vitro. a (Left panel) Immunocytochemical staining of choline acetyl transferase (ChAT) (Millipore, dilution 1:50) in BMMs stimulated with M-CSF and RANKL and (right panel) control staining with secondary antibody (AB) (biotinylated rabbit-antigoat IgG) only. Scale bars = 100 μm. b–g Effect of nAChR ligands on osteoclastogenesis. Images in the bottom panel are representative of corresponding experiments; scale bar = 100 μm; b nicotine; c epibatidine; d PNU282987; e nicotine after pretreatment with 100 μM mecamylamine; f mecamylamine; g alpha-bungarotoxin. BTX alphabungarotoxin; TRAP tartrate-resistant acidic phosphatase