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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Nicotinic acetylcholine receptors modulate osteoclastogenesis

Fig. 4

Nicotinic AChR ligands block the differentiation of TRAP-positive mononuclear cells in vitro. a Nicotine; b epibatidine; c PNU282987; d mecamylamine; e alpha-bungarotoxin; f effect of nicotine on cytoxicity as assessed by MTT assay; g percentage of live (Annexin V low/7-AAD low), apoptotic (Annexin V high/7-AAD low) and dead (Annexin V high/7-AAD high) cells with flow cytometry plots showing representative experiments (right panels). hj Effect of nicotine on percentage of cells at the end of the in vitro osteoclastogenesis assay staining positive for marker: g F4/80; h CD11b; i GR1 low/CD11b+. 7-AAD 7-Aminoactinomycin-D, OD optical density, TRAP tartrate-resistant acidic phosphatase

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