Fig. 5

Osteoclastogenesis is partly inhibited in nAChR subunit –/– mice. a, b Osteoclastogenesis in WT, α7–/–, β2–/– and β4–/– mice with or without 500 μg/ml nicotine treatment. Images in the right panel are representative of the corresponding experiments; scale bars = 100 μm. c, e Bone volume/tissue volume; d, f trabecular density; g trabecular thickness; h trabecular separation; i number of osteoclasts/bone perimeter; j osteoclast surface/bone surface. TRAP tartrate-resistant acidic phosphatase, WT wild-type