Fig. 4From: Cartilage-specific deletion of ephrin-B2 in mice results in early developmental defects and an osteoarthritis-like phenotype during aging in vivoImpaired vascularization of ephrin-B2 (EFNB2)Col2knockout (KO) cartilage conditional mice at postnatal day P15. Representative immunohistochemical and histochemical sections of proximal tibia, and histograms of EFNB2fl/fl (n = 7) and EFNB2Col2KO cartilage conditional (n = 7) mice at P15 for vascular endothelial growth factor (VEGF) (a-c)), von Kossa (d-f) and tartrate resistant alkaline phosphatase (TRAP) (g-i). Sections were counterstained with methyl green. Original magnification × 400. e Scale bar at 100 μm. f %BV/TV % bone volume/tissue volume, Tb Thickness trabecular thickness. Arrows indicate positive staining (a, b, g, h) and trabecular thickness (d, e). Data are expressed as the mean ± standard error of the mean and P values were determined using the Mann–Whitney U testBack to article page