Fig. 5

Bone abnormalities of the ephrin-B2 (EFNB2)^Col2knockout (KO) cartilage conditional mice. a Representative macroscopic and radiographic EFNB2^fl/fl (n = 6) and EFNB2^Col2KO cartilage conditional (n = 6) mice at postnatal day P21. In the radiographs, the lower portion of EFNB2^Col2KO cartilage conditional mice shows osteopenia (arrow) compared to EFNB2^fl/fl mice. b Bone mineral density measurements of EFNB2^fl/fl and EFNB2^Col2KO cartilage conditional mice at P21, 8 weeks and 1 year (n = 8–10 for each category). Micro-computed tomography (μCT) analysis of the distal femur (c) and proximal tibia (d) (n = 8) at 8 weeks. e Representative histological sections of the proximal tibia stained with Safranin O; original magnification × 400, scale bar at 100 μm. % BV/TVo % bone volume/tissue volume, Tb trabecular thickness. Data are expressed as mean ± standard error of the mean and the P values were determined by Mann–Whitney U test