Fig. 9

Locomotor phenotype of some ephrin-B2 (EFNB2)^Col2knockout (KO) cartilage conditional mice is related to an abnormal corticospinal tract. a-f Brain tracing experiments to visualize the corticospinal path with biotinylated dextran amines (BDA) performed in 6-week-old EFNB2^fl/fl (n = 2) and EFNB2^Col2KO cartilage conditional (n = 3) mice. Representative photomicrographs of the cervical spinal cord of EFNB2^fl/fl (a, c, d) and EFNB2^Col2KO cartilage conditional mice (b, e, f) at the level of the fifth cervical vertebra. g Percentage of BDA-labeled fibers in the contralateral and ipsilateral hemispheres. Arrowheads indicate the injection site (a, b) and labeled fibers (c-f). Scale bars at 100 μm (a, d). Data are expressed as the mean ± standard error of the mean and the P values were determined by Mann–Whitney U test