Fig. 3

T-cell effects and synthesis of inflammatory factors by sorted CD271⁺ and CD271⁻ stromal cells (SC). a T-cell proliferation at 72 h and cytokine production at 24 h in supernatants of SC/T-cell cocultures stimulated with CD3/CD28 Dynabeads. Data are representative of paired CD271⁺ and CD271⁻ synovial SCs from nine different synovial tissue donors cocultured with T cells from nine allogeneic donors (mean ± standard error of the mean are indicated). *p < 0.05 by analysis of variance with Friedman and Dunn’s multiple comparisons tests. b Synthesis of inflammatory mediators in 24-h culture supernatants of paired CD271⁺ and CD271⁻ synovial SC cultures as quantified by multiplex enzyme-linked immunosorbent assay. Graphics represent triplicate samples from three patients with OA (mean ± standard deviation). *p < 0.05 by Wilcoxon test. NS not significant, IL interleukin, IFN interferon, MMP matrix metalloproteinase, MCP-1 monocyte chemoattractant protein 1, VEGF vascular endothelial growth factor