Fig. 4
From: CD271+ stromal cells expand in arthritic synovium and exhibit a proinflammatory phenotype
Analysis of CD271+ and CD271− stromal cell (SC) implants in immunodeficient mice. a Engrafted SCs were identified in Matrigel implants by immunoperoxidase labeling with antihuman nuclei or isotype control (CTRL) (hematoxylin counterstaining; bar, 100 μm). b Identification on infiltrating myeloid cells by phycoerythrin (PE)-CD11b antibody (red) and 4′,6-diamidino-2-phenylindole (DAPI) counterstaining (blue), PE–immunoglobulin G2b isotype control is also shown. c Representative images of sections stained with DAPI from CD271− and CD271+ Matrigel implants (left panel). Quantitative analysis of cellularity (DAPI-positive nuclei per square millimeter) in CD271− and CD271+ implants (right panel). Data are from six different OA synovial membranes, sorted as CD271+ or CD271− SCs and implanted into NSG mice. *p = 0.03 by Wilcoxon test