Fig. 2

Representative 3D views showing reconstructed imaging of FDA and PI stained medial femoral condyle. a Mid-sagittal plane of reconstructed condylar images, including the pixel-labeling technique using 1s and 0s (inset). b Mid-coronal plane of reconstructed condylar images. c Reconstructed apical view of condyle with the pixel-labeling technique (inset). Scale bars indicate 250 μm. d Confocal z-stack image series showing sample sections from apex of condyle (0 μm) down into the medial femoral condyle cartilage thickness (28 μm). Estimated thickness of stained cartilage without loss of central cellularity was 14 μm, which included the superficial and upper intermediate cartilage zones. Scale bars indicate 400 μm. FDA fluorescein diacetate, PI propidium iodide