Fig. 1
Colony-stimulating factor-1 (CSF-1), IL-34 and CSF1 receptor (CSF1R) are expressed in synovial tissue from patients with rheumatoid arthritis (RA) and psoriatic arthritis (PsA). a Quantification of relative IL-34, CSF-1 and CSF1R mRNA expression in synovial tissue from 6 patients with RA and 6 with PsA. Quantitative PCR data are shown as relative quantity (RQ), as described in “Methods”. Data are presented as scatter plots, where each plot represents an individual value, bars represent the mean, and error bars indicate the standard error of the mean (SEM). b, c Immunohistochemical analyses of RA, PsA and OA synovial tissue stained with control rabbit, anti-IL34 (b) and anti-CSF1 antibodies (c). d Quantitative analysis of IL-34 and CSF-1 staining in synovial tissue. Synovial sections from 15 patients with RA, 15 with PsA and 7 patients with osteoarthritis (OA) were stained with antibodies against IL-34 and CSF-1 antibodies as above, and the integrated optical density (IOD/mm 2) corrected for cellularity was calculated by digital image analysis. Each plot represents an individual value, bars represent the mean, and error bars indicate the SEM. *P < 0.05