Table 1 Oligonucleotide primers used for real-time polymerase chain reaction
Target gene | Primers (forward and reverse) | Product size (bp) | GenBank accession number | Primer efficiency (%) |
|---|---|---|---|---|
RPS9 | GATTACATCCTGGGCCTGAA ATGAAGGACGGGATGTTCAC | 161 | NM_001013 | 94.5 |
ADAMTS4 | CTGCCTACAACCACCG GCAACCAGAACCGTCC | 293 | NM_005099.4 | 99.1 |
ADAMTS5 | GCACTTCAGCCACCATCAC AGGCGAGCACAGACATCC | 187 | NM_007038.3 | 92.4 |
MMP13 | TCACGATGGCATTGCT GCCGGTGTAGGTGTAGA | 277 | NM_002427 | 94.5 |
S100A8 | TAGAGACCGAGTGTCCTCA CGCCCATCTTTATCACCAGA | 126 | NM_002964.4 | 93.4 |
S100A9 | CCATCATCAACACCTTCCACCA CTGCTTGTCTGCATTTGTGTCC | 179 | NM_002965.3 | 91.4 |