Fig. 2

SjS-associated miRNAs are upregulated in CD14⁺ monocytes. Expression verification by qRT-PCR for combined microarray and validation cohorts including HC (circle, n = 17), SjS (pSjS closed square, n = 21; sSjS open square, n = 9), SLE (triangle, n = 17), and RA (diamond, n = 18) samples. Horizontal lines and bars represent mean ± SEM. Dotted line indicates cutoff value determined by ROC curve analyses (Additional file 4: Figure S2). True positive rate (TPR, sensitivity) = true positive/(true positive + false negative), true negative rate (TNR, specificity) = true negative/(true negative + false positive). ^* P < 0.05, ^*** P < 0.001, ^**** P < 0.0001 by one-way ANOVA with Bonferroni post tests. HC healthy control, pSjS primary Sjögren’s syndrome, RA rheumatoid arthritis, SLE systemic lupus erythematosus, SjS Sjögren’s syndrome, sSjS secondary Sjögren’s syndrome