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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: HM71224, a novel Bruton’s tyrosine kinase inhibitor, suppresses B cell and monocyte activation and ameliorates arthritis in a mouse model: a potential drug for rheumatoid arthritis

Fig. 3

HM71224 inhibits human B cell activation. a Healthy primary human CD19+ B cells (n = 3) were activated with anti-IgM F(ab’)2 fragments in the presence of increasing concentrations of HM71224. Phosphorylation of Bruton’s tyrosine kinase (Btk) and PLCγ2 in human B cells was inhibited in a dose-dependent manner. The immunoblot shown is representative of three independent experiments. b HM71224 inhibits the surface expression of CD40, CD69, and CD86 by activated B cells. c Percentage of Btk sites on human peripheral blood mononuclear cells (PBMCs) occupied by HM71224, as measured by a biotinylated probe. *P < 0.001 vs. 0 nM HM71224. ERK extracellular signal-regulated kinase

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