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Table 2 Monocyte and PMN phenotypes

From: Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis

Phenotype Patients with AAV Healthy controls P value
Monocytes (% of leukocytes) 4.4 ± 0.3 4.8 ± 0.24 n.s.
(109/L, reference range <1,1) 0.33 ± 0.02   
Lymphocytes (% of leukocytes) 20 ± 1.2 31 ± 1.2 <0.0001
(109/L, reference range 1.1–4.8) 0.74 ± 0.05   
PMN (% of leukocytes) 39 ± 1.3 28 ± 1.1 <0.0001
(109/L, reference range 1.7–8.0) 5.9 ± 0.3   
CD16+CD10+ (% of PMN) 84 ± 1.1 82 ± 1.2 n.s.
CD16dimCD10- (% of PMN) 8.9 ± 0.8 6.8 ± 0.6 0.0109
CD177+ (% of PMN) 55 ± 2.4 47 ± 1.9 0.0075
CD10+CD16+ PMN    
CD88+ (geoMFI) 442 ± 14 409 ± 15 n.s
CD62L+ (geoMFI) 1,277 ± 37 1,269 ± 41 n.s.
CD11c+(geoMFI) 558 ± 14 596 ± 16 n.s
CD10+CD16dim PMN    
CD88+ (geoMFI) 391 ± 8.2 381 ± 7.6 n.s
CD62L+ (geoMFI) 450 ± 23 457 ± 22 n.s
CD11c+(geoMFI) 354 ± 7.2 428 ± 10 <0.0001
Monocytes    
CD88+ (geoMFI) 162 ± 2.5 165 ± 2.5 n.s
CD62L+ (geoMFI) 770 ± 29 651 ± 37 <0.0001
CD11c+(geoMFI) 1,645 ± 63 2,079 ± 78 <0.0001
  1. The frequencies of monocytes, lymphocytes, polymorphonuclear leukocytes (PMN), CD10+CD16+ (mainly segment nucleated neutrophils) and CD10-CD16dim (suggested as a marker for newly released neutrophils) were investigated in healthy controls (n = 109) and patients (n = 105) using flow cytometry. In addition, the level of surface expression of CD88 (C5aR), CD62L and CD11c was studied on CD10+ CD16+ PMN, CD10 + CD16dim PMN and monocyte populations, as a measurement of activation and reported as geometric mean fluorescence intensity (geoMFI). The two-sided Mann-Whitney test was used to calculate the level of significance. Values are reported as mean ± SEM. AAV anti-neutrophil cytoplasmic antibodies associated vasculitides, n.s. not significant