Fig. 2

IL-17A inhibits differentiation of cultured calvarial osteoblasts. Osteoblasts are differentiated in the presence or absence of IL-17A (5 or 50 ng/ml). a Representative Von Kossa staining at days 21 and 28 of differentiation (n = 3 independent experiments). b Representative mRNA expression of alkaline phosphatase and osteocalcin relative to undifferentiated calvarial osteoblasts (day 0) by qRT-PCR over the course of osteoblast differentiation (n = 3 independent experiments) (*p < 0.05, **p < 0.01, ***p < 0.001 for IL-17A at 5 ng/ml; ⁺ p < 0.05, ⁺⁺ p < 0.01, ⁺⁺⁺⁺ p < 0.0001 for IL-17A at 50 ng/ml). c mRNA expression of alkaline phosphatase and osteocalcin by gene array in mature calvarial osteoblasts at day 28, differentiated in the continuous presence of IL-17A (50 ng/ml) compared to day 28 control calvarial osteoblasts