Fig. 3From: Reduced glutathione as a physiological co-activator in the activation of peptidylarginine deiminaseReduced glutathione (GSH) induces PAD activity in synovial fluid (SF). Microtitre plates were coated with human fibrinogen and incubated for 3 h at RT with a pool of SF from five RA patients supplemented with different combinations of CaCl2 (10 mM), GSH (10 mM) or EDTA (25 mM). Citrullination of fibrinogen was measured using mAb 20B2 as the detecting antibody. Bars and error bars represent the mean and SEM of triplicate measurementsBack to article page