Fig. 5
From: Reduced glutathione as a physiological co-activator in the activation of peptidylarginine deiminase
Effect of glutathione reductase inhibition on the catalytic activity of peptidylarginine deiminase (PAD) released from cells. a Microtitre plates coated with human fibrinogen were incubated for 3 h at RT with leucocytes isolated from three healthy donors in media without PMA, media with 15 nM PMA alone or media with 15 nM PMA in combination with 50 μM of the glutathione reductase inhibitor (GRI) 2-AAPA. Citrullination of fibrinogen was detected using mAb 20B2 and optical density (OD) values are shown as the average and upper range of triplicate measurements. b PAD2 concentration in cell supernatants is shown as the average and upper range of duplicate measurements. c Catalytic activity of PAD in cell supernatants supplemented with 5 mM CaCl2 and 25 mM of reduced glutathione (GSH) shown as the average and upper range of duplicate OD measurements using mAb 20B2 as a detector of citrullinated fibrinogen. PMA phorbol 12-myristate 13-acetate