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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Antibodies to carbamylated α-enolase epitopes in rheumatoid arthritis also bind citrullinated epitopes and are largely indistinct from anti-citrullinated protein antibodies

Fig. 1

Human anti-citrullinated protein antibodies (ACPA) cross-react with carbamylated epitopes. a Native (n), in vitro citrullinated (Cit) and in vitro carbamylated (Carb) samples of recombinant human α-enolase and purified human fibrinogen were subjected to western blot using affinity-purified anti-CCP2 IgG (ACPA IgG) and CCP2-depleted column flow-through IgG (FT IgG), obtained from a pool of 38 rheumatoid arthritis (RA) serum samples. b Affinity-purified anti-CEP-1 IgG and the corresponding column FT IgG fractions from five patients with RA were tested for reactivity to the carb-CEP-1 peptide in ELISA; antibody levels are presented as optical density (OD)

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Arthritis Research & Therapy

ISSN: 1478-6362

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