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Table 3 Characteristics of the antibodies used for this study

From: Different expression of β subunits of the KCa1.1 channel by invasive and non-invasive human fibroblast-like synoviocytes

Target Host Vendor (catalog number; manufacturer location) Conjugation Clone Use
Primary antibodies
 Actin Rabbit Sigma-Aldrich (A2066; St. Louis, MO, USA) WB
 Actin Mouse Sigma-Aldrich (A3853) AC-40 WB
 Cadherin-11 Mouse Thermo Fisher Scientific (MA1-06306; Rockford, IL, USA) 16A FC
 CD44 Mouse Abcam (ab187571; Cambridge, MA, USA) Alexa Fluor 488 MEM85 FC, EP
 KCa1.1α Mouse Antibodies, Inc. (NeuroMab, 75-022; UC Davis/NIH NeuroMab Facility, Davis, CA, USA) L6/60 WB
 KCa1.1α Rabbit EMD Millipore (AB5228; Billerica, MA, USA) FC
 KCa1.1β1 Rabbit Novus Biologicals (NBP1-33484; Littleton, CO, USA) WB
 KCa1.1β2 Mouse Antibodies, Inc. (NeuroMab, 75-087; UC Davis/NIH NeuroMab Facility) N53/32 WB
 KCa1.1 pan-β3 Rabbit Abcam (ab137041) EPR9543(B) WB
 KCa1.1 β3a, βc, βd, βe Mouse Rockland Immunochemicals (200-301-E96; Limerick, PA, USA) S40B-18 WB
 KCa1.1β4 Mouse Antibodies, Inc. (NeuroMab, 75-086; UC Davis/NIH NeuroMab Facility) L18A/3 WB
 MMP-2 Mouse BioLegend (634802; San Diego, CA, USA) F14P4D3 FC
 Podoplanin Rat BioLegend (337003) Phycoerythrin NC-08 FC
Secondary antibodies
 Mouse IgG Goat LI-COR Biosciences (926-32210; Lincoln, NE, USA) Infrared-800 WB
 Mouse IgG1 Goat Thermo Fisher Scientific (A-21127) Alexa Fluor 555 FC
 Rabbit IgG Donkey LI-COR Biosciences (926-68023) Infrared-680 WB
 Rabbit IgG Goat Abcam (ab150079) Alexa Fluor 647 FC
  1. EP electrophysiology, FC flow cytometry, IgG immunoglobulin G, KCa calcium-activated potassium channel, MMP matrix metalloproteinase, WB Western blot analysis