|
Primary antibodies
|
|
Actin
|
Rabbit
|
Sigma-Aldrich (A2066; St. Louis, MO, USA)
|
–
|
–
|
WB
|
|
Actin
|
Mouse
|
Sigma-Aldrich (A3853)
|
–
|
AC-40
|
WB
|
|
Cadherin-11
|
Mouse
|
Thermo Fisher Scientific (MA1-06306; Rockford, IL, USA)
|
–
|
16A
|
FC
|
|
CD44
|
Mouse
|
Abcam (ab187571; Cambridge, MA, USA)
|
Alexa Fluor 488
|
MEM85
|
FC, EP
|
|
KCa1.1α
|
Mouse
|
Antibodies, Inc. (NeuroMab, 75-022; UC Davis/NIH NeuroMab Facility, Davis, CA, USA)
|
–
|
L6/60
|
WB
|
|
KCa1.1α
|
Rabbit
|
EMD Millipore (AB5228; Billerica, MA, USA)
|
–
|
–
|
FC
|
|
KCa1.1β1
|
Rabbit
|
Novus Biologicals (NBP1-33484; Littleton, CO, USA)
|
–
|
–
|
WB
|
|
KCa1.1β2
|
Mouse
|
Antibodies, Inc. (NeuroMab, 75-087; UC Davis/NIH NeuroMab Facility)
|
–
|
N53/32
|
WB
|
|
KCa1.1 pan-β3
|
Rabbit
|
Abcam (ab137041)
|
–
|
EPR9543(B)
|
WB
|
|
KCa1.1 β3a, βc, βd, βe
|
Mouse
|
Rockland Immunochemicals (200-301-E96; Limerick, PA, USA)
|
–
|
S40B-18
|
WB
|
|
KCa1.1β4
|
Mouse
|
Antibodies, Inc. (NeuroMab, 75-086; UC Davis/NIH NeuroMab Facility)
|
–
|
L18A/3
|
WB
|
|
MMP-2
|
Mouse
|
BioLegend (634802; San Diego, CA, USA)
|
–
|
F14P4D3
|
FC
|
|
Podoplanin
|
Rat
|
BioLegend (337003)
|
Phycoerythrin
|
NC-08
|
FC
|
|
Secondary antibodies
|
|
Mouse IgG
|
Goat
|
LI-COR Biosciences (926-32210; Lincoln, NE, USA)
|
Infrared-800
|
–
|
WB
|
|
Mouse IgG1
|
Goat
|
Thermo Fisher Scientific (A-21127)
|
Alexa Fluor 555
|
–
|
FC
|
|
Rabbit IgG
|
Donkey
|
LI-COR Biosciences (926-68023)
|
Infrared-680
|
–
|
WB
|
|
Rabbit IgG
|
Goat
|
Abcam (ab150079)
|
Alexa Fluor 647
|
–
|
FC
|
