Table 3 Characteristics of the antibodies used for this study
Target | Host | Vendor (catalog number; manufacturer location) | Conjugation | Clone | Use |
|---|---|---|---|---|---|
Primary antibodies | |||||
Actin | Rabbit | Sigma-Aldrich (A2066; St. Louis, MO, USA) | – | – | WB |
Actin | Mouse | Sigma-Aldrich (A3853) | – | AC-40 | WB |
Cadherin-11 | Mouse | Thermo Fisher Scientific (MA1-06306; Rockford, IL, USA) | – | 16A | FC |
CD44 | Mouse | Abcam (ab187571; Cambridge, MA, USA) | Alexa Fluor 488 | MEM85 | FC, EP |
KCa1.1α | Mouse | Antibodies, Inc. (NeuroMab, 75-022; UC Davis/NIH NeuroMab Facility, Davis, CA, USA) | – | L6/60 | WB |
KCa1.1α | Rabbit | EMD Millipore (AB5228; Billerica, MA, USA) | – | – | FC |
KCa1.1β1 | Rabbit | Novus Biologicals (NBP1-33484; Littleton, CO, USA) | – | – | WB |
KCa1.1β2 | Mouse | Antibodies, Inc. (NeuroMab, 75-087; UC Davis/NIH NeuroMab Facility) | – | N53/32 | WB |
KCa1.1 pan-β3 | Rabbit | Abcam (ab137041) | – | EPR9543(B) | WB |
KCa1.1 β3a, βc, βd, βe | Mouse | Rockland Immunochemicals (200-301-E96; Limerick, PA, USA) | – | S40B-18 | WB |
KCa1.1β4 | Mouse | Antibodies, Inc. (NeuroMab, 75-086; UC Davis/NIH NeuroMab Facility) | – | L18A/3 | WB |
MMP-2 | Mouse | BioLegend (634802; San Diego, CA, USA) | – | F14P4D3 | FC |
Podoplanin | Rat | BioLegend (337003) | Phycoerythrin | NC-08 | FC |
Secondary antibodies | |||||
Mouse IgG | Goat | LI-COR Biosciences (926-32210; Lincoln, NE, USA) | Infrared-800 | – | WB |
Mouse IgG1 | Goat | Thermo Fisher Scientific (A-21127) | Alexa Fluor 555 | – | FC |
Rabbit IgG | Donkey | LI-COR Biosciences (926-68023) | Infrared-680 | – | WB |
Rabbit IgG | Goat | Abcam (ab150079) | Alexa Fluor 647 | – | FC |