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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Single-cell antibody nanowells: a novel technology in detecting anti-SSA/Ro60- and anti-SSB/La autoantibody-producing cells in peripheral blood of rheumatic disease patients

Fig. 2

Profiling anti-SSA/Ro60- and anti-SSB/La-producing B cells using single-cell antibody nanowell technology. a Representative micrographs of cells (bright field [BF]) in nanowells labeled with calcein (live cells) and CD19-Alexa Fluor 488 (AF488). Micrographs of matching microarray by microengraving showing detection signals for immunoglobulin G (IgG)-AF647, anti-SSA/Ro60-AF488, and anti-SSB/La-AF550. b Profiling of autoantibody-producing B cells in nanowells (n = 9 healthy control subjects, n = 13 pSS/SLE patients). Data extracted by image processing with GenePix software were used to identify the appropriate signals. The data were correlated with the nanowell image data in which nanowells contained a single cell positive for both calcein and CD19. The percentage of CD19+ B cells producing IgG, anti-SSA/Ro60, anti-SSB/La, anti-SSA/Ro60 in IgG isotype, and anti-SSB/La in IgG isotype was determined by using the count of positive signals from wells with single cells and the total number of wells with single cells. *p < 0.05, **p < 0.01 by unpaired t test

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Arthritis Research & Therapy

ISSN: 1478-6362

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