Skip to main content
Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Single-cell antibody nanowells: a novel technology in detecting anti-SSA/Ro60- and anti-SSB/La autoantibody-producing cells in peripheral blood of rheumatic disease patients

Fig. 3

Quantifying the concentration of anti-SSA/Ro60 and anti-SSB/La produced by individual B cells. A standard curve for the antibodies produced by each cell was constructed by applying a series of concentrations of corresponding fluorochrome-conjugated antihuman immunoglobulin G (IgG) (e.g., 1 nM to 10 μM) to the set of replicate microarrays and then measuring the mean fluorescence intensity of captured anti-SSA/Ro60 and anti-SSB/La as a function of concentration using data processing of micrographs of microarrays. a Concentration of each signal spot from nanowells with single live CD19+ B cell producing IgG-specific anti-SSA/Ro60. ^Seronegative patients for anti-SSA/Ro60 autoantibody. b Combined concentrations of individual positive signal spots (live CD19+ B cells secreting IgG-specific anti-SSA/Ro60 autoantibody) from patients and healthy control subjects. c Concentration of each signal spot from nanowells with single live CD19+ B cell producing IgG-specific anti-SSB/La. ^Seronegative patients for anti-SSB/La autoantibody. d Combined concentrations of individual positive signal spots (live CD19+ B cells secreting IgG-specific anti-SSB/La autoantibody) from patients and healthy control subjects. X-axis in (a) and (c) denotes healthy and patient coded personal identification. *p < 0.05. NS not significant by unpaired t test

Back to article page

Arthritis Research & Therapy

ISSN: 1478-6362

Contact us