Fig. 4

Matrix metalloproteinase-3 (MMP-3) with elevated Agrocybe cylindracea galectin (ACG)/Jacalin signals produced in local lesions reflects the degree of sialylation. a Synovial fluid MMP-3 was measured by Western blot analysis after immunoprecipitation. Patients with RA (lanes 1–3) and patients with OA (lanes 4–6). b Representative lectin signals derived from synovial MMP-3 (mean ± SD). c The structure of T and sialyl-T antigen. Jacalin recognizes the former. d Jacalin and ACG signals compared before and after sialic acid digestion (mean ± SD). e The estimated basic structure of MMP-3 O-glycan. ABA Agaricus bisporus agglutinin, UDA Urtica dioica agglutinin, ACA Amaranthus caudatus agglutinin, WGA wheat germ agglutinin