Fig. 2

Reactivity of anti-prothrombin (aPT) mouse monoclonal (a) and anti-annexin V (aAnV) rabbit polyclonal antibodies (b) with phospholipids (PL) and PL-binding proteins by line immunoassay (LIA): aPT and aAnV were run in the LIA alone or together with blood donor serum, bovine serum albumin (BSA) and PT and AnV, respectively. As the positive reaction control a mixture of human IgG and IgM was immobilized. The control band was only revealed when anti-human IgG coupled to horseradish peroxidase was used as secondary antibody conjugate. a 1, 1.0 mg/L aPT; 2, 1.0 mg/L aPT + 10 mg/L PT; 3, 1.0 mg/L aPT + 10 mg/L BSA; 4, 1.0 mg/L aPT + 30 μL serum (1/33); and 5, 30 μL serum (1/33). b 1, 5.0 mg/L aAnV; 2, 5.0 mg/L aAnV + 10 mg/L AnV; and 3, 5.0 mg/L aAnV + 10 mg/L BSA