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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Notochordal cell conditioned medium (NCCM) regenerates end-stage human osteoarthritic articular chondrocytes and promotes a healthy phenotype

Fig. 1

Outline of experimental approach. Human articular chondrocytes (Ch) collected from healthy and osteoarthritic (OA) cartilage tissues were expanded in vitro and then cultured in pellets (phase I culture). Resulting tissues were then exposed to interleukin 1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) for 3 days (d) and then cultured for an additional 2 weeks (phase II culture) in medium containing 2 % FBS-supplemented notochordal cell conditioned medium (NCCM) or in control medium (2 % FBS-supplemented ADMEM/F12, (CTR)). Pellets generated from OA chondrocytes were cultured in NCCM or CTR medium without any pro-inflammatory treatment. TGF transforming growth factor

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