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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Notochordal cell conditioned medium (NCCM) regenerates end-stage human osteoarthritic articular chondrocytes and promotes a healthy phenotype

Fig. 3

Biochemical analyses of pellets generated with osteoarthritic (OA) chondrocytes. a DNA contents (left) and sulfated glycosaminoglycan (GAG) contents normalized to the DNA content (GAG/DNA) (right) of pellets after the phase I culture (baseline) and after 2, 7, and 14 additional days of culture in control (ctr) medium or notochordal cell conditioned medium (NCCM) (see Fig. 1 for the experimental design). Levels are expressed as fold-difference from those measured at baseline. Data are means ± SD (n indicates the number of cartilage donors; for each donor two to three experimental replicates were analyzed); *p < 0.05 from baseline; °p < 0.05 from ctr (same culture time). b GAG/DNA content of pellets generated by healthy chondrocytes at baseline (n = 4, same as those in Fig. 2, > 55 years of age) and OA chondrocytes after phase II culture in ctr medium and NCCM; *significant difference at p < 0.05; n.s. difference not significant

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