Fig. 6

Effect of soluble sialic acid-binding immunoglobulin-type lectin (sSiglec)-9 on the messenger RNA (mRNA) expression of matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, and interleukin (IL)-6 in fibroblast-like synoviocytes (FLS) derived from synovial tissue of patients with rheumatoid arthritis. FLS were stimulated with tumor necrosis factor (TNF)-α in the presence or absence of sSiglec-9 (0–20 ng/ml) for 12 h. Relative mRNA expression of MMP-1, MMP-3, MMP-13, and IL-6 standardized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was determined by real-time polymerase chain reaction. a, b, c, d sSiglec-9 treatment had no effect on the TNF-α-stimulated induction of MMP-1, MMP-3, MMP-13, and IL-6 mRNA expression