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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Inflammatory conditions partly impair the mechanically mediated activation of Smad2/3 signaling in articular cartilage

Fig. 4

The effect of mechanical compression carried out in presence of inflammatory conditions on the expression of TGF-β receptors. The influence of 3 and 12 MPa dynamic mechanical compression, carried out with IL-1β (squares) or with OAS-CM (triangles) or without inflammatory conditions (dots), on the relative expression of bAlk5 (a) and bTgfbr2 (b). Dynamic mechanical compression with 3 MPa as well as with 12 MPa potently upregulated bAlk5 in conditions without inflammatory conditions. However, in the presence of IL-1β, 3 MPa as well as 12 MPa mechanical compression had no influence on bAlk5 expression. In the presence of OAS-CM, only 3 MPa compression upregulated bAlk5. Dynamic mechanical compression had no influence on bTgfbr2 expression in conditions without inflammatory conditions. It had also no influence on bTgfbr2 expression in the presence of IL-1β. However, in the presence of OAS-CM, 12 MPa compression induced a downregulation of bTGfbr2. Data are expressed as a grouped column scatter of multiple repeats with displayed mean (each point represents individual experimental repeat on material isolated from a different animal, N = 4). * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. CTR control, GAPDH glyceraldehyde 3-phosphate dehydrogenase, IL-1β interleukin-1 beta, OAS-CM osteoarthritic synovium-conditioned medium, TGF-β transforming growth factor beta

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