Table 1 Primers used for QPCR
Gene | Full gene name | Ref seq | Product length | Forward 5′-- > 3′ | Reverse 5′-- > 3′ |
|---|---|---|---|---|---|
bAlk5 | bovine transforming growth factor, beta receptor 1 | NM_174621.2 | 75 | CAGGACCACTGCAATAAAATAGAACTT | TGCCAGTTCAACAGGACCAA |
bGapdh | bovine glyceraldehyde 3-phosphate dehydrogenase | NM_001034034.2 | 90 | CACCCACGGCAAGTTCAAC | TCTCGCTCCTGGAAGATGGT |
bJunB | bovine jun B proto-oncogene | NM_001075656.1 | 139 | CCTTCTACCACGACGACTCA | CCGGGTGCTTTGAGATTTCG |
bRps14 | bovine ribosomal protein S14 | NM_001077830.2 | 125 | CATCACTGCCCTCCACATCA | TTCCAATCCGCCCAATCTTCA |
bSerpine1 | bovine plasminogen activator inhibitor type 1 | NM_174137.2 | 55 | CGAGCCAGGCGGACTTC | TGCGACACGTACAGAAACTCTTGA |
bSmad7 | bovine SMAD family member 7 | NM_001192865.1 | 72 | GGGCTTTCAGATTCCCAACTT | CTCCCAGTATGCCACCACG |
bTgfb1 | bovine transforming growth factor, beta 1 | NM_001166068.1 | 80 | GGTGGAATACGGCAACAAAATCT | GCTCGGACGTGTTGAAGAAC |
bTgfbr2 | bovine transforming growth factor beta receptor II | NM_001159566.1 | 141 | GGCTGTCTGGAGGAAGAATGA | GTCTCTCCGGACCCCTTTCT |
hALK5 | human transforming growth factor, beta receptor 1 | NM_001306210.1 | 65 | CGACGGCGTTACAGTGTTTCT | CCCATCTGTCACACAAGTAAA |
hGAPDH | human glyceraldehyde 3-phosphate dehydrogenase | NM_001289745.1 | 143 | ATCTTCTTTTGCGTCGCCAG | TTCCCCATGGTGTCTGAGC |
hRPS27 | human ribosomal protein S27 | NM_001177413.1 | 90 | TGGCTGTCCTGAAATATTATAAGGT | CCCCAGCACCACATTCATCA |
hSERPINE1 | human plasminogen activator inhibitor type 1 | NM_000602.4 | 213 | GTCTGCTGTGCACCATCCCCCATC | TTGTCATCAATCTTGAATCCCATA |
hSMAD7 | human SMAD family member 7 | NM_001190821.1 | 131 | CCTTAGCCGACTCTGCGAACTA | CCAGATAATTCGTTCCCCCTGT |
hTGFB1 | human transforming growth factor, beta 1 | NM_000660.5 | 59 | GAGGTCACCCGCGTGCTA | TGCTTGAACTTGTCATAGATTTCGTT |