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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion

Fig. 2

Effect of interaction between synoviocytes and PBMC on Th17 differentiation and IL-17 secretion. Healthy PBMC were cultured alone or in co-culture with RA synoviocytes at a 5:1 ratio for 48 h, in the presence or absence of PHA (5 μg/ml). The transwell system was used in co-cultures to inhibit cell–cell contact and fixed synoviocytes or fixed PBMC were used. PBMC were recovered after 48 h and stained for the surface markers CD3 and CD4, and intracellular IL-17A. The percentage of CD3 + CD4 + IL-17A + is represented for each experiment (a). IL-17A production was measured in supernatants after 48 h, by ELISA (b and c). Each linked dot plot represents one experiment in the different conditions. *Compares the culture conditions (PBMC alone, co-culture or transwell) with and without PHA. #Compares the activation state (control or PHA) in the culture conditions. *# p ≤ 0.05. IL interleukin, PBMC peripheral blood mononuclear cells, PHA phytohemagglutinin, RA rheumatoid arthritis

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