Fig. 3

Confirmation in an autologous system and with other mesenchymal cell types. Healthy PBMC were cultured alone or in co-culture with RA synoviocytes, ASC or HUVEC at a 5:1 ratio for 48 h, in the presence or absence of PHA (5 μg/ml). Levels of IL-17 and IL-6 were measured in the supernatants after 48 h by ELISA (a). PBMC from RA patients were cultured alone or in co-culture with autologous synoviocytes or from other patients, at the ratio 5:1 for 48 h, in the presence or absence of PHA (5 μg/ml). Levels of IL-17 and IL-6 were measured in the supernatants after 48 h by ELISA. Values are individual results (b). ASC adipose-derived stem cells, HUVEC human umbilical vein endothelial cells. IL interleukin, PBMC peripheral blood mononuclear cells, PHA phytohemagglutinin, RA rheumatoid arthritis