Skip to main content
Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion

Fig. 5

The major role of podoplanin in the high IL-17 secretion. Healthy or RA PBMC were pre-incubated 4 h with or without human anti-pdpn or irrelevant antibody before co-culture with synoviocytes or autologous synoviocytes, respectively, for 48 h, in the presence of PHA (5 μg/ml). For siRNA, RA synoviocytes were transfected without (control) or with siRNA (pdpn, PPIB or mock) and then used in co-culture with healthy PBMC, in the presence of PHA, during 48 h. Levels of IL-17, IL-1β and IL-6 were measured in the supernatants after 48 h by ELISA. The concentration of IL-17 in the different conditions of culture is represented (a). The expression of the genes pdpn and PPIB compared to control (control = 1) (b) is represented as well as the percentage of cytokine inhibition compared to control in the heterologous system (c) and in the autologous system (d). * p ≤ 0.05 compared to control. IL interleukin, PBMC peripheral blood mononuclear cells, PHA phytohemagglutinin, RA rheumatoid arthritis, pdpn podoplanin, PPIB peptidylpropyl isomerase B, siRNA small interfering RNA

Back to article page