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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: H3K27me3 demethylases regulate in vitro chondrogenesis and chondrocyte activity in osteoarthritis

Fig. 4

Impact of trimethylated histone 3 lysine 27 (H3K27me3) demethylase inhibition on mesenchymal stem cell (MSC) behaviour. a NANOG expression in monolayer MSCs treated for 24 h with GSK-J4 and chondrogenic medium compared with chondrogenic medium alone (n = 4 individuals, 4 technical replicates per condition). b PAI1 and JMJD3 expression were decreased in monolayer MSCs treated for 1 h with GSK-J4 and chondrogenic medium compared with chondrogenic medium alone (n = 4 individuals, 4 technical replicates per condition). c H3K27me3 staining (green) in MSCs cultured for 24 h in control medium, chondrogenic medium or chondrogenic medium plus GSK-J4. Cell cytoskeleton/actin (phalloidin, red) and nuclear (4′,6-diamidino-2-phenylindole, blue) staining. **p ≤ 0.01, ***p ≤ 0.001. Data are presented as individual data points for biological replicates showing mean ± 95 % CI. a and b Analysis of variance with Dunnett’s correction for multiple comparisons

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