Fig. 4

Detection of GFP⁺ cells in synovium of bone marrow chimeric mice following joint surface injury. a Schematic diagram showing experimental design for the data shown in this figure and Fig. 5. b Representative images of immunofluorescent staining for GFP (green) and the proliferation marker CldU (red) in control and injured knee joint synovium. Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Scale bars = 25 μm. The insets show magnified views of the areas within dotted rectangles. BM bone marrow, S synovium, C capsule, P patella, F femur. c–e Quantification of GFP and CldU single- and double-positive cell populations in the synovium. Data are shown as individual data points from three mice, with horizontal lines indicating mean values. **p < 0.01; ***p < 0.001. Act-eGFP C57BL/6-Tg14(act-EGFP)OsbY01 mice, CldU chlorodeoxyuridine, GFP green fluorescent protein, IdU iododeoxyuridine