Fig. 5

Proliferation of host slow-cycling cells in synovium following joint surface injury. The schematic diagram of the experimental design is shown in Fig. 4a. a Representative images of immunofluorescent staining for iododeoxyuridine (IdU, green) and chlorodeoxyuridine (CldU, red) in control and injured knee joint synovium. Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale bars = 25 μm. The insets show magnified views of the areas within dotted rectangles. S synovium, C capsule, P patella, F femur. b, c Quantification of nucleoside analogue-labelled cells in the synovium. Data are shown as individual data points from three mice, with horizontal lines indicating mean values. *p < 0.05; **p < 0.01