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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Collagen triple helix repeat containing 1 is a new promigratory marker of arthritic pannus

Fig. 4

Co-localization of cells expressing synovial markers. Sagittal plane histological sections of the medial aspect of inflamed knee joints were stained with hematoxylin-eosin (a), macrophage-specific calcium-binding protein IBA1 (b, j), alpha smooth muscle actin (α-SMA) (c, d, l), collagen triple helix repeat containing 1 (CTHRC1) (e, f, k), cadherin CDH11 (gi). Sections were counterstained with hematoxylin or alcian blue. Meniscus (m) was used to align images; femur (f), tibia (t). Area of the pannus-meniscus junction labeled with yellow arrowheads (c, e, g) are zoomed in at panels d, f, and h. Representative immunohistochemical (IHC) images at panels il show the area of pannus-meniscus junction at higher × 40 objective magnification. At this structure, cells positive for CDH11, CTHRC1 and α-SMA IHC staining demonstrated a similar gradient distribution with maximal signal at the meniscus fibrocartilage-pannus border, while macrophage-specific IBA1 showed dispersed localization. Scale bars are 100 μm (ah) and 25 μm (il)

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