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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Collagen triple helix repeat containing 1 is a new promigratory marker of arthritic pannus

Fig. 6

Time-lapse microscopy analysis of fibroblast migration under collagen triple helix repeat containing 1 (CTHRC1) treatment. NIH 3T3 fibroblasts seeded onto μ-slides were treated with rhCTHRC1 at 1000 ng/ml and observed using time-lapse microscopy. (a) Cell trajectories derived from stacks of images taken every 15 minutes during migration were presented as Rose plots to compare cell motility parameters. (b) Each cell trajectory was analyzed for accumulated distance (Ad), Euclidean distance (Ed), directness (Ed/Ad), and cell velocity (Ad/time). (c) Average values for 20–40 cells for each condition were compared. Solid black columns represent cells treated with rhCTHRC1, gray columns represent untreated cells. (d) Cell length was defined as a Euclidean distance between the most distant points, usually between the front of lamellipodia and the end of cell tail. Cell length is shown by a white bar. Phase contrast images of cells in the beginning and after 12 hours CTHRC1 treatment are presented. Scale bar is 20 μm. (e) Cell length was measured at the beginning and the end of observation period. Data represent four similar but independent experiments. Statistical significance was calculated using Student’s t test: *p < 0.05; **p < 0.01 for comparison of treatment versus no treatment

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