Fig. 2

PAD4 is not required for TNF-α-driven lung citrullination. Lung lysates from TNF⁺PAD4^+/+ and TNF⁺PAD4^-/- mice were exposed to Rh-PG, followed by gel electrophoresis, imaging of Rh-PG, then staining with brilliant blue to detect total protein. a Representative gels. b Total Rh-PG signal was normalized to total protein with average and SEM graphed. Lysates were subjected to western blot using the F95 antibody to detect citrullinated proteins and gel electrophoresis with brilliant blue to detect total protein. c Representative western blot (left) and brilliant blue-stained gel (right). d Total F95 signal was normalized to total protein with average and SEM graphed. For all panels, n = 9 littermate pairs. PAD4 peptidylarginine deiminase 4, TNF-α tumor necrosis factor alpha