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Table 2 Expression of UPR genes in response to treatment with thapsigargin and tunicamycin in human chondrocytes

From: The unfolded protein response genes in human osteoarthritic chondrocytes: PERK emerges as a potential therapeutic target

 

Tg

Tm

 

Normal

p value

OA

p value

Normal

p value

OA

p value

ERN1

5.29 ± 1.49

0.063

7.15 ± 0.61

≤0.001

3.76 ± 1.12

0.089

4.12 ± 0.36

≤0.001

PERK

3.54 ± 0.34

0.005

2.52 ± 0.21

≤0.001

2.18 ± 0.48

0.089

1.59 ± 0.12

0.006

ATF6B

1.05 ± 0.06

0.446

0.82 ± 0.08

0.074

1.07 ± 0.30

0.837

0.86 ± 0.12

0.325

CREB3L2

1.15 ± 0.26

0.606

1.38 ± 0.34

0.307

1.15 ± 0.20

0.502

1.20 ± 0.16

0.271

GRP78

38.54 ± 6.27

0.009

35.68 ± 4.45

≤0.001

19.18 ± 2.40

0.005

16.74 ± 2.31

≤0.001

CHOP

22.08 ± 6.72

0.052

25.64 ± 6.49

0.012

13.66 ± 3.06

0.025

6.34 ± 1.66

0.023

DNAJB9

15.04 ± 4.37

0.049

12.33 ± 1.77

≤0.001

9.56 ± 2.86

0.009

7.76 ± 0.75

≤0.001

SREBP2

0.79 ± 0.11

0.165

0.84 ± 0.11

0.200

0.51 ± 0.09

0.013

0.68 ± 0.09

0.015

  1. The normal (n = 4) and osteoarthritic (OA; n = 6–12) chondrocytes were treated for 20 hours with thapsigargin (Tg; 50 nM) and tunicamycin (Tm; 500 ng/ml) and processed for qPCR. Each untreated control was assigned an arbitrary value of 1. The effect of each treatment was evaluated as fold change over control and expressed as mean values ± SEM. p values were assessed by the one-sample t test, comparing the treated chondrocytes to the untreated controls (arbitrary value of 1); values ≤0.05 were considered significant. Comparison between normal and OA was assessed by the Mann-Whitney test; data revealed no significant differences (p values not shown)
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Arthritis Research & Therapy

ISSN: 1478-6362

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