Fig. 2

Activation of the CXCL10 promoter in synovial cell micromasses by LPS, TNF-α and IL-1β. Synovial micromasses of four OA patients were transduced with the CXCL10p-fluc-RPL22p-rluc dual-luciferase construct and stimulated for 6 h with (a) 100 ng/ml LPS, (b) 10 ng/ml TNF-α or (c) 10 ng/ml IL-1β and compared to the unstimulated medium condition. The signal ratio was calculated as fireflyRLU/renillaRLU +/- SD. Micromasses are depicted as individual points and different colours represent different patients. Statistical analysis between medium and stimulated condition was performed by Student’s t test and comparisons within individual patient samples were calculated by two-way ANOVA. *P < 0.05, **P < 0.01. IL-1β interleukin-1 beta, LPS lipopolysaccharide, TNF-α tumour necrosis factor alpha