Fig. 4

Production of IL-10 by transduced micromasses. Micromasses from synovial cell suspensions were transduced with lentiviral vectors coding for PGK-luciferase, PGK-IL10 or CXCL10p-IL10 after formation of a synovial lining. Subsequently, the micromasses were stimulated with medium containing 100 ng/ml LPS (a) or 10 ng/ml TNF-α (b). The IL-10 concentration in the supernatant after 24 h was measured using a multiplex ELISA assay. Concentrations below 1 pg/ml were included in the statistical analysis, but are shown as 1 pg/ml in the Figure. An insufficient number of micromasses could be generated from patients 10 and 12 to determine the response to TNF-α. The medium, LPS- and TNF-α-stimulated groups were compared by one-way ANOVA. *P < 0.05, **P < 0.01. IL-10 interleukin-10, LPS lipopolysaccharide, TNF-α tumour necrosis factor alpha