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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Apoptotic cell infusion treats ongoing collagen-induced arthritis, even in the presence of methotrexate, and is synergic with anti-TNF therapy

Fig. 3

Apoptotic cell injection favors pro-regulatory properties to antigen presenting cells (APC). Plasmacytoid dendritic cells (pDC), conventional DC (cDC) and macrophages (macro) were isolated from the spleen of collagen-induced arthritis (CIA) mice receiving or not receiving apoptotic cells (+ApoCell) and were quantified (a), evaluated for costimulatory and class II molecules (CD40, IAIE, respectively) expression ex vivo or after stimulation by adequate toll-like receptor ligand (b, c), and cultured with naïve allogeneic CD4+ T cells to evaluate T regulatory cell (Treg) induction (d). Data are shown as means from seven independent experiments; each experiment includes five mice per group; *p < 0.05, **p < 0.01 (parametric paired Student t test) (a-c). d Data from individual mouse performed in triplicate plus mean (black bar) from one of three representative experiments; ***p < 0.001 (parametric unpaired t test). MFI mean fluorescence intensity

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