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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: A2A adenosine receptor upregulation correlates with disease activity in patients with systemic lupus erythematosus

Fig. 1

Messenger RNA (mRNA) and protein expression of adenosine receptors (ARs) in human lymphocytes from patients with systemic lupus erythematosus (SLE) and healthy subjects. a Relative AR mRNA levels were determined by real-time reverse transcriptase-polymerase chain reaction. Experiments were performed in duplicate with lymphocytes obtained from individual patients with SLE (n = 80) and healthy subjects (n = 80), and data are shown as mean ± SEM. b Western blot analysis showing immunoblot signals of ARs in one patient with SLE and one healthy subject, representative of blots obtained with lymphocytes from 80 patients with SLE and 80 healthy control subjects. β-actin was used as a loading control. c Densitometric analysis of AR expression in human lymphocytes from patients with SLE (n = 80) and healthy subjects (n = 80) indicated as a ratio of β-actin (loading control). Data are expressed as the mean ± SEM of densitometric analysis results obtained from the indicated number of subjects. * p < 0.01 versus control group by one-way analysis of variance with Dunnett’s test

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